DIS3 anticorps (AA 62-304)

N° du produit ABIN6719579

L’anticorps Lapin Polyclonal anti-DIS3 a été validé pour WB, ELISA, IP, IF, ICC et FACS. Il convient pour détecter DIS3 dans des échantillons de Humain et Rat.

Aperçu rapide pour DIS3 anticorps (AA 62-304) (ABIN6719579)

Antigène: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DIS3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-DIS3 anticorps

Épitope: AA 62-304

Fonction: Anti-DIS3 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-DIS3 Antibody (ABIN6719579). Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human DIS3 recombinant protein (Position: Q62-A304).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999. 2. Rozenblum, E., Vahteristo, P., Sandberg, T., Bergthorsson, J. T., Syrjakoski, K., Weaver, D., Haraldsson, K., Johannsdottir, H. K., Vehmanen, P., Nigam, S., Golberger, N., Robbins, C., and 12 others. A genomic map of a 6-Mb region at 13q21-q22 implicated in cancer development: identification and characterization of candidate genes. Hum. Genet. 110: 111-121, 2002. 3. Tomecki, R., Kristiansen, M. S., Lykke-Andersen, S., Chlebowski, A., Larsen, K. M., Szczesny, R. J., Drazkowska, K., Pastula, A., Andersen, J. S., Stepien, P. P., Dziembowski, A., Jensen, T. H. The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L. EMBO J. 29: 2342-2357, 2010.

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur DIS3

Antigène: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Autre désignation: DIS3

Sujet:

Synonyms: Exosome complex exonuclease RRP44, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, DIS3, KIAA1008, RRP44

Tissue Specificity: Widely expressed.

Background: Exosome complex exonuclease RRP44 or Dis3 is an enzyme that in humans is encoded by the DIS3 gene. Its protein product is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the nucleus of eukaryotic cells. By genomic sequence analysis, this gene is mapped to chromosome 13q21-q22. Expression of human DIS3 could partially complement Dis3 depletion in yeast.

Poids moléculaire: 109 kDa

UniProt: Q9Y2L1